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https://mp.weixin.qq.com/s/qwYdgiyQsYvZJiXeHN4Bew

近日，广东省科学院王振宇团队在Plant Physiol发表了题为《》的研究论文，揭示了大豆伪应答调节蛋白3b和转录因子ABF3调节脱落酸依赖性干旱胁迫反应。

昼夜节律系统在促进作物有效应对和适应周围环境波动的能力方面发挥着关键作用。尽管人们对伪应答调节蛋白（PRRs）的理解越来越深入，并且它们参与了多种生物过程的调节，包括昼夜节律、开花的光周期控制和对非生物胁迫的反应，但大豆中与这些因子相关的转录网络仍然有待于进一步揭示。

在这项研究中，作者提供了证据表明：GmPRR3b作为调节大豆昼夜节律时钟、干旱胁迫反应和脱落酸（ABA）信号通路的关键介质的重要性。DNA亲和纯化测序和转录组数据的综合分析确定了795个由GmPRR3b直接调控的推定靶基因。其中，570个基因与干旱反应显著相关，8个基因同时参与ABA的生物合成和信号通路。

值得注意的是，GmPRR3b通过抑制脱落酸反应元件结合因子3（GmABF3）的表达，在大豆干旱反应的负调控中发挥了关键作用。此外，GmABF3的过表达表现出更强的耐受干旱条件的能力，并且它还恢复了GmPRR3b过表达导致的干旱敏感表型。一致的是，对植物中GmPRR3b基因表达的操纵和基因组编辑的研究揭示了对干旱胁迫的不同反应。

作者的研究结果共同提供了可靠的证据，强调了GmPRR3b-GmABF3模块在增强大豆抗旱性方面的重大作用。此外，GmPRR3b的转录网络为该基因与植物适应不同环境条件的基本生物过程之间的复杂相互作用提供了有价值的见解。

The circadian system plays a pivotal role in facilitating the ability of crop plants to respond and adapt to fluctuations in their immediate environment effectively. Despite the increasing comprehension of PSEUDO-RESPONSE REGULATORs (PRRs) and their involvement in the regulation of diverse biological processes, including circadian rhythms, photoperiodic control of flowering, and responses to abiotic stress, the transcriptional networks associated with these factors in soybean (Glycine max (L.) Merr.) remain incompletely characterized. In this study, we provide empirical evidence highlighting the significance of GmPRR3b as a crucial mediator in regulating the circadian clock, drought stress response, and abscisic acid (ABA) signaling pathway in soybeans. A comprehensive analysis of DNA affinity purification sequencing and transcriptome data identified 795 putative target genes directly regulated by GmPRR3b. Among them, a total of 570 exhibited a significant correlation with the response to drought, and eight genes were involved in both the biosynthesis and signaling pathways of ABA. Notably, GmPRR3b played a pivotal role in the negative regulation of the drought response in soybeans by suppressing the expression of abscisic acid responsive element-binding factor 3 (GmABF3). Additionally, the overexpression of GmABF3 exhibited an increased ability to tolerate drought conditions, and it also restored the hypersensitive phenotype of the GmPRR3b overexpressor. Consistently, studies on the manipulation of GmPRR3b gene expression and genome editing in plants revealed contrasting reactions to drought stress. The findings of our study collectively provide compelling evidence that emphasizes the significant contribution of the GmPRR3b-GmABF3 module in enhancing drought tolerance in soybean plants. Moreover, the transcriptional network of GmPRR3b provides valuable insights into the intricate interactions between this gene and the fundamental biological processes associated with plant adaptation to diverse environmental conditions.

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